AMPK beta 1 (PRKAB1) pSer182 Rabbit Polyclonal Antibody

CAT#: AP20874PU-N

AMPK beta 1 (PRKAB1) pSer182 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-AMPKbeta1 antibody (Cat.-No.: AP20874PU-N) in extracts from COS7 cells

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  Immunohistochemistry (IHC) analyzes of p-AMPK1 antibody (Cat.-No.: AP20874PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-AMPKbeta1 protein only when phosphorylated at Ser182.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 35 kDa
• Gene Name: Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1)
• Database Link:
  Entrez Gene 19079 MouseEntrez Gene 83803 RatEntrez Gene 5564 Human
• Background: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic alpha subunit and regulatory beta and gamma subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl- CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKalpha1 and AMPKalpha2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKbeta1 encodes a 271 amino acid protein and human AMPKbeta2 encodes a 272 amino acid protein. The human AMPKgamma1 gene encodes a 331 amino acid protein.
• Synonyms: PRKAB1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway