EIF4E pSer209 Rabbit Polyclonal Antibody

CAT#: AP20885PU-N

EIF4E pSer209 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-eIF4E antibody (Cat.-No.: AP20885PU-N) in extracts from NIH/3T3 FBS cells.

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  Immunohistochemistry (IHC) analyzes of p-eIF4E antibody (Cat.-No.: AP20885PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-eIF4E protein only when phosphorylated at Ser209.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 25 kDa
• Gene Name: Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1
• Database Link:
  Entrez Gene 13684 MouseEntrez Gene 117045 RatEntrez Gene 1977 Human
• Background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
• Synonyms: eIF-4F 25 kDa subunit, EIF-4E, EIF4EL1, EIF4F

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway