FOXO3 pSer253 Rabbit Polyclonal Antibody

CAT#: AP20889PU-N

FOXO3 pSer253 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-FOXO3A antibody (Cat.-No.: AP20889PU-N)

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  Immunohistochemistry (IHC) analyzes of Phospho-FOXO3A antibody (pSer253) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human FOXO3A around the phosphorylation site of Serine 253.
• Specificity: This antibody detects endogenous levels of p-FOXO3A protein when phosphorylated at Ser253.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 97 kDa
• Gene Name: Homo sapiens forkhead box O3 (FOXO3), transcript variant 1
• Database Link:
  Entrez Gene 56484 MouseEntrez Gene 294515 RatEntrez Gene 2309 Human
  UniProt ID O43524
• Background: FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. It recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. FOXO3a has been shown to induce cell growth arrest to effect tumor suppression and FOXO3A genotype is strongly associated with human longevity.
• Synonyms: FOXO3A, AF6q21 protein

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Endometrial cancer, Neurotrophin signaling pathway, Non-small cell lung cancer