Histone H2A.X (H2AFX) pSer139 Rabbit Polyclonal Antibody

CAT#: AP20894PU-N

Histone H2A.X (H2AFX) pSer139 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-Histone H2A.X antibody (Cat.-No.: AP20594PU-N) in extracts from 293 neocarzinostatin cells

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  Immunohistochemistry (IHC) analyzes of p-Histone H2A.X antibody (AP20894PU-N) in paraffin-embedded human malignant lymphoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-Histone H2A.X protein only when phosphorylated at Ser139.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 15 kDa
• Gene Name: Homo sapiens H2A histone family member X (H2AFX)
• Database Link:
  Entrez Gene 15270 MouseEntrez Gene 500987 RatEntrez Gene 3014 Human
• Background: Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. The Histone H2A.X gene is located in close proximity to the porphobilinogen deaminase (PBGD) gene in both mouse and human, and maps to 9 A5.2 and 11q23.3, respectively. H2A.X differs from the other members of the H2A family by the presence of a highly conserved C-terminal motif. It is rapidly phosphorylated in response to ionizing radiation and plays an important role in the recognition and repair of DNA double stranded breaks. The phosphorylated form of H2A.X, designated γ-H2A.X, forms nuclear foci at the heavy chain constant region of cells involved in class switch recombination (CSR), a region-specific DNA reaction that replaces one immunoglobulin heavy chain constant region gene with another. The phosphorylated γ-H2A.X is also thought to initiate subsequent repair factors, including Rad50, Rad51 and BRCA1.
• Synonyms: H2a/x, H2AFX, H2AX

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus