AMPK alpha 1 (PRKAA1) pSer486 Rabbit Polyclonal Antibody
CAT#: AP20922PU-N
AMPK alpha 1 (PRKAA1) pSer486 rabbit polyclonal antibody, Aff - Purified
Other products for "PRKAA1"
Specifications
Product Data | |
Applications | IHC |
Recommended Dilution | Immunohistochemistry on Paraffin Sections: 1/50-1/200. |
Reactivities | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Synthetic phosphopeptide derived from human AMPKα1 around the phosphorylation site of Serine 486. |
Specificity | This antibody detects endogenous levels of AMPK alpha-1 protein only when phosphorylated at Ser486. The antibody does not cross-react with phosphorylated AMPK alpha-2 or other related proteins. |
Formulation | Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% Sodium Azide |
Concentration | 1.0 mg/ml |
Purification | Affinity Chromatography using epitope-specific immunogen (> 95% by SDS-PAGE) |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Predicted Protein Size | ~ 63 kDa |
Gene Name | Homo sapiens protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant 1 |
Database Link | |
Background | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory beta and gamma subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl- CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKalpha1 and AMPKalpha2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKbeta1 encodes a 271 amino acid protein and human AMPKbeta2 encodes a 272 amino acid protein. The human AMPKgamma1 gene encodes a 331 amino acid protein. Human AMPKgamma2 and AMPKgamma3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. |
Synonyms | AMPK1, AMPK alpha-1 chain |
Reference Data | |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy |
Documents
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