Estrogen Receptor 1 (ESR1) pSer104 Rabbit Polyclonal Antibody

CAT#: AP20937PU-N

Estrogen Receptor 1 (ESR1) pSer104 rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry (IHC) analyzes of p-ER-a (pSer104) pAb in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: p-ER-a (pSer104) pAb detects endogenous levels of p-ER-a protein
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1,0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1
• Database Link:
  Entrez Gene 13982 MouseEntrez Gene 24890 RatEntrez Gene 2099 Human
• Background: The estrogen receptor-alpha (ER) is a broadly expressed transcription factor which controls a number of genes involved in cellular differentiation and proliferation. ER contains two transcriptionally active domains: the N-terminal domain (AF1) is constitutively active, while the C-terminal domain (AF-2) is hormone-dependent. ER is subject to posttranslational modification, including phosphorylation of serine residues 104, 106, 118, and 167. This antibody specifically recognizes human estrogen receptor-alpha when phosphorylated at serine 167, a site which influences AF-1 dependent transcriptional activity. This phosphorylation is catalyzed by the MAPK pathway, potentially through the 90 kDa ribosomal S6 kinase (pp90rsk1). Serine 167 is also a consensus Akt substrate.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors