LIM Kinase 1 (LIMK1) (+LIMK2) Rabbit Polyclonal Antibody

CAT#: AP20986PU-N

LIM Kinase 1 (LIMK1) (+LIMK2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of LIMK1/2 antibody (Cat.-No.: AP20986PU-N) in extracts from COS7 cells.

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  Immunohistochemistry (IHC) analyzes of LIMK1/2 antibody (Cat.-No.: AP20986PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of LIMK1/2 protein.
  (region surrounding Pro514)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 72 kDa
• Gene Name: Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1
• Database Link:
  Entrez Gene 16885 MouseEntrez Gene 65172 RatEntrez Gene 3984 Human
• Background: Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation.
  Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.
• Synonyms: LIMK, LIM domain kinase 1, LIMK-1, EC=2.7.11.1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton