NF-kB p65 (RELA) Rabbit Polyclonal Antibody

CAT#: AP21046PU-N

NF-kB p65 (RELA) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry (IHC) analyzes of NFkB-p65 pAb in paraffin-embedded human lung adenocarcinoma tissue.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
  Immunohistochemistry: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: NFkB-p65 pAb detects endogenous levels of NFkBp65 protein.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1,0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1
• Database Link:
  Entrez Gene 19697 MouseEntrez Gene 309165 RatEntrez Gene 5970 Human
• Background: p65 is a subunit of the nuclear factor kappa B. The transcription factor NFKB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFKB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFKB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFKB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.
• Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway