SMAD2 Rabbit Polyclonal Antibody

CAT#: AP21054PU-N

SMAD2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Smad2 antibody (Cat.-No.: AP21054PU-N) in extracts from RAW264.7cells.

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  Immunohistochemistry (IHC) analyzes of Smad2 antibody (Cat.-No.: AP21054PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Smad2 protein.
  (region surrounding Tyr216)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 60 kDa
• Gene Name: Homo sapiens SMAD family member 2 (SMAD2), transcript variant 2
• Database Link:
  Entrez Gene 17126 MouseEntrez Gene 29357 RatEntrez Gene 4087 Human
• Background: Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptorregulated Smad's, activin/TGFâ receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling.
• Synonyms: SMAD family member 2, SMAD-2, SMAD 2, MADH2, MAD homolog 2, MADR2, Mad-related protein 2, JV18-1

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway