ATF1 Rabbit Polyclonal Antibody

CAT#: AP21087PU-N

ATF1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ATF-1 antibody in extracts from COLO cells.

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  Immunohistochemistry (IHC) analyzes of ATF-1 antibody in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human ATF1.
• Specificity: This antibody detects endogenous levels of ATF-1 protein.
  (region surrounding Ile57)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Predicted Protein Size: ~ 29 kDa
• Gene Name: Homo sapiens activating transcription factor 1 (ATF1)
• Database Link:
  Entrez Gene 11908 MouseEntrez Gene 466 Human
• Background: Eukaryotic gene transcription is regulated by sequencespecific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKAinduced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.
• Synonyms: ATF-1, Activating transcription factor 1, Protein TREB36

Reference Data

• Protein Families: Druggable Genome, Transcription Factors