RAD51 Rabbit Polyclonal Antibody

CAT#: AP21099PU-N

RAD51 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Rad51 antibody (Cat.-No.: AP21099PU-N) in extracts from LOVO cells.

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  Immunohistochemistry (IHC) analyzes of Rad51 antibody (Cat.-No.: AP21099PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Rad51 protein.
  (region surrounding Thr309)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 42 kDa
• Gene Name: Homo sapiens RAD51 recombinase (RAD51), transcript variant 1
• Database Link:
  Entrez Gene 19361 MouseEntrez Gene 5888 Human
• Background: Rad51 is a recombinase that is essential for homologous recombination and double-strand break repair. It binds to single and double-stranded DNA, and has DNA-dependent ATPase activity. Rad51 unwinds duplex DNA and forms helical nucleoprotein filaments in a manner that is controlled through the action of DNA helicases that counteract nucleofilament formation. Rad51 interacts with BRCA1 and BRCA2, and also plays a role in p53-mediated signalling. Interaction of Rad51 with Chk1 kinase may occur in response to Chk1 phosphorylation.
• Synonyms: RAD51A, RECA

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Homologous recombination, Pancreatic cancer, Pathways in cancer