SRC Rabbit Polyclonal Antibody

CAT#: AP21124PU-N

SRC rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of c-Src antibody (Cat.-No.: AP21124PU-N) in extracts from HeLa cells.

• 

  Immunohistochemistry (IHC) analyzes of c-Src antibody (Cat.-No.: AP21124PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of c-Src protein.
  (region surrounding Ser69)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1,0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 60 kDa
• Gene Name: Homo sapiens SRC proto-oncogene, non-receptor tyrosine kinase (SRC), transcript variant 1
• Database Link:
  Entrez Gene 20779 MouseEntrez Gene 83805 RatEntrez Gene 6714 Human
  UniProt ID P12931
• Background: The major translational products of each of the eight members of the Src gene family identified to date are membraneassociated tyrosine protein kinases that lack transmembrane and external amino acid sequences, thereby distinguishing this group from the receptor class of tyrosine kinases. Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr. The major Src gene encoded protein, c-Src (also designated pp60Src, Src p60 and proto-oncogene tyrosine protein kinase Src) is expressed in a broad range of tissue and cell types, although the highest levels of c-Src are detected in neuronal tissues and platelets. c-Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions.
• Synonyms: c-Src, pp60c-src

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Stem cell relevant signaling - JAK/STAT signaling pathway
• Protein Pathways: Adherens junction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, GnRH signaling pathway, Tight junction, VEGF signaling pathway