SLP76 (LCP2) Rabbit Polyclonal Antibody

CAT#: AP21127PU-N

SLP76 (LCP2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of SLP-76 antibody (Cat.-No.: AP21127PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of SLP-76 antibody (Cat.-No.: AP21127PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of SLP-76 protein.
  (region surrounding Gly122)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 75 kDa
• Gene Name: Homo sapiens lymphocyte cytosolic protein 2 (LCP2)
• Database Link:
  Entrez Gene 16822 MouseEntrez Gene 155918 RatEntrez Gene 3937 Human
• Background: Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production. When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response. Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1. The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation.
• Synonyms: LCP2, Lymphocyte cytosolic protein 2, SLP-76 tyrosine phosphoprotein, SLP76

Reference Data

• Protein Pathways: Fc epsilon RI signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway