VCP Rabbit Polyclonal Antibody

CAT#: AP21142PU-N

VCP rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of VCP antibody (Cat.-No.: AP21142PU-N) in extracts from 3T3 cells.

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  Immunohistochemistry (IHC) analyzes of VCP antibody (Cat.-No.: AP21142PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of VCP protein.
  (region surrounding Ala346)
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 88 kDa
• Gene Name: Homo sapiens valosin containing protein (VCP)
• Database Link:
  Entrez Gene 116643 RatEntrez Gene 269523 MouseEntrez Gene 7415 Human
• Background: Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinsons Disease.
• Synonyms: 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein