PAF49 (CD3EAP) Rabbit Polyclonal Antibody

CAT#: AP21165PU-N

PAF49 (CD3EAP) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CD3EAP antibody (Cat.-No.: AP21165PU-N) in extracts from A549 cells.

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  Immunohistochemistry (IHC) analyzes of CD3EAP antibody (Cat.-No.: AP21165PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of CD3EAP protein.
  (region surrounding Glu474)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Predicted Protein Size: ~ 55 kDa
• Gene Name: Homo sapiens CD3e molecule associated protein (CD3EAP), transcript variant 2
• Database Link:
  Entrez Gene 10849 Human
• Background: CD3EAP, also known as ASE1, CAST or PAF49, is a 510 amino acid protein that localizes to the nucleus and belongs to the eukaryotic RPA34 RNA polymerase subunit family. Existing as two alternatively spliced isoforms, CD3EAP functions as a component of the Pol I complex, specifically exhibiting DNA-dependent RNA polymerase activity and effectively catalyzing the the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. Isoform 2 of CD3EAP may be a component of the T cell receptor (TCR) complex and is subject to tyrosine phosphorylation in response to TCR stimulation. Both isoforms of CD3EAP are subject to DNA damage-dependent phosphorylation, probably by ATM or ATR.
• Synonyms: ASE1, CAST, PAF49, A34.5

Reference Data

• Protein Families: Transcription Factors