RPB11 (POLR2J) Rabbit Polyclonal Antibody

CAT#: AP21166PU-N

RPB11 (POLR2J) rabbit polyclonal antibody, Aff - Purified


USD 370.00

2 Weeks*

Size
    • 100 ug

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Other products for "POLR2J"

Specifications

Product Data
Applications WB
Recommended Dilution Western blot: 1/500 - 1/1000.
Reactivities Human, Mouse
Host Rabbit
Clonality Polyclonal
Specificity This antibody detects endogenous levels of POLR2J1 protein.
(region surrounding Asp24)
Formulation Phosphate buffered saline (PBS), pH 7.2
State: Aff - Purified
State: Liquid purified Ig fraction
Preservative: 0.05% Sodium azide
Concentration 1.0 mg/ml
Purification Affinity chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Predicted Protein Size ~ 13 kDa
Gene Name Homo sapiens RNA polymerase II subunit J (POLR2J)
Background Four independent genes encoding various variants of the hRPB11 subunit of Homo sapiens RNA polymerase II were revealed in human chromosome 7. Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated. On the basis of the scheme, some assumptions on the pathways of evolution of separate human genes and the mechanisms of generation of protein diversity in higher eukaryotes were made.
Synonyms RNA polymerase II subunit B11-a, POLR2J1, RPB11a
Reference Data
Protein Families Transcription Factors
Protein Pathways Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

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