MDFI Rabbit Polyclonal Antibody

CAT#: AP21195PU-N

MDFI rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MDFI antibody (Cat.-No.: AP21195PU-N) in extracts from Jurkat cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of MDFI protein.
  (region surrounding Pro134)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 25 kDa
• Gene Name: Homo sapiens MyoD family inhibitor (MDFI), transcript variant 3
• Database Link:
  Entrez Gene 17240 MouseEntrez Gene 4188 Human
  UniProt ID Q99750
• Background: MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.
• Synonyms: MyoD family inhibitor

Reference Data

• Protein Families: Transcription Factors