Carbonic Anhydrase I (CA1) (Erythrocytes) Rabbit Polyclonal Antibody

CAT#: AP21226BT-N

Carbonic Anhydrase I (CA1) (Erythrocytes) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  Working dilutions in non-precipitating antibody-binding techniques: 1/2,000-1/10,000.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Carbonic Anhydrase I isolated and purified from Human Erythrocytes.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The antibody recognizes Carbonic Anhydrase I from Human Erythrocytes.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized Hyperimmune IgG fraction
  Molar radio: Biotin/ IgG ~4.9
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens carbonic anhydrase I (CA1), transcript variant 1
• Database Link:
  Entrez Gene 759 Human
• Synonyms: Carbonic anhydrase I, Carbonate dehydratase I, Carbonic anhydrase B, CA1, CAB, CA-I

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism