Ceruloplasmin (CP) Rabbit Polyclonal Antibody

CAT#: AP21233BT-N

Ceruloplasmin (CP) rabbit polyclonal antibody, Biotin


USD 410.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
<i>Working dilutions in non-precipitating antibody-binding techniques:</i> 1/1,000-1/10,000.
Reactivities Human
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Ceruloplasmin isolated and purified from Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The antibody recognizes Ceruloplasmin from Human serum.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: Biotin
State: Lyophilized Hyperimmune IgG fraction
Label: N-Hydroxysuccinimidobiotin
Molar radio: Biotin/IgG: ~5.6
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water; Prepare working dilutions by adding sterile phosphate buffered saline (PBS, ph 7.2) and preferably use the same day.
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Biotin
Storage Store the antibody lyophilized at 2-8°C
Reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens ceruloplasmin (ferroxidase) (CP), transcript variant 1
Background Caeruloplasmin is a copper binding plasma glycoprotein consisting of a single polypeptide chain. It has a molecular weight of 132kDa and occurs in plasma at a concentration of 150-500 mg/L. Although the physiological functions of Caeruloplasmin are not fully understood it is implicated in a number of possible functions. 95% of plasma copper is bound to Caeruloplasmin, implying some involvement in copper transport. Caeruloplasmin has been described as both an oxidant and an antioxidant, its exact role or roles in this respect are not clear. Plasma levels of Caeruloplasmin are elevated after inflammation and trauma and it is therefore classified as an acute phase protein. The measurement of Caeruloplasmin in plasma is useful in the diagnosis of Wilson’s disease, where levels are greatly reduced. Caeruloplasmin is extremely labile in plasma and rapidly degrades to 115kDa and 19kDa fragments. The age of plasma samples should therefore be taken into account when interpreting immunological analysis of caeruloplasmin.
Synonyms Ferroxidase, CP
Reference Data
Protein Families Druggable Genome
Protein Pathways Porphyrin and chlorophyll metabolism

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.