Ceruloplasmin (CP) Rabbit Polyclonal Antibody

CAT#: AP21233SU-N

Ceruloplasmin (CP) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: In precipitating techniques as Immunoelectrophoresis and single or Double Radial Immunodiffusion to identify the presence of alpha-2 Ceruloplasmin in Human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody binding techniques if proper controls are included.
  Recommended Dilutions:
  Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
  Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Ceruloplasmin is isolated from pooled Human serum by sequential precipitation and purified by Ion Exchange Chromatography and Affinity Chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to alpha-2 Ceruloplasmin. In Immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal Human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-2 ceruloplasmin. No reaction is obtained with any other plasma protein component or serum.
  Cross-reactivity: The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: State: Serum
  State: Lyophilized, Delipidated, heat inactivated, stable whole antiserum
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore by adding 1 ml sterile distilled water.
• Concentration: lot specific
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ceruloplasmin (ferroxidase) (CP), transcript variant 1
• Database Link:
  Entrez Gene 1356 Human
• Background: Alpha-2 ceruloplasmin is copper containing alpha-2 glycoprotein with a molecular weight of 160,000. Its mean concentration in plasma of adults is 32 mg/100ml. Elevated levels are observed in pregnant women, in patients with infectious diseases and the nephritic syndrome. More than 90% of the plasma copper is bound to this protein.
• Synonyms: Ferroxidase, CP
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Porphyrin and chlorophyll metabolism