Butyrylcholinesterase (BCHE) Rabbit Polyclonal Antibody

CAT#: AP21238AF-N

Butyrylcholinesterase (BCHE) rabbit polyclonal antibody, Azide Free

Product Images

Formalin-Fixed, Paraffin-Embedded Human brain, cortex stained with Cholinesterase Antibody at 1/200 after heat-induced antigen retrieval.

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
  Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/40,000.
  Immunohistochemistry on Paraffin Sections: 1/200.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Butyrylcholinesterase isolated and purified from Human serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The antibody recognizes Butyrylcholinesterase from Human Serum.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized Hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens butyrylcholinesterase (BCHE)
• Database Link:
  Entrez Gene 590 Human
• Background: Butyrylcholinesterase is synthetizised in the liver, and is predominantly found in serum, liver and pancreas. This enzyme is a tetrameric glycoprotein (molecular mass of 350 kDa), and consists of four subunits. Defects in Butyrylcholinesterase are the cause of a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics.
• Synonyms: Butyrylcholine esterase, Pseudocholinesterase, BCHE, CHE1

Reference Data

• Protein Families: Druggable Genome, Transmembrane