Alanine aminotransferase 1 / ALT1 Rabbit Polyclonal Antibody

CAT#: AP21264BT-N

Alanine aminotransferase 1 / ALT1 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/2,000-1/50,000.
• Reactivities: Porcine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Glutamic-Pyruvic Transaminase isolated and purified from porcine heart.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~ 4.2
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Background: Alanine Transaminase [glutamic pyruvate transaminase - GPT] participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Pyridoxal phosphate is a known cofactor. Expression is induced by glucocorticoids. This enzyme is present in a high concentration in liver tissue and it's release into the blood indicates liver disease or damage.
• Synonyms: GPT, AAT1, GPT1