Alanine aminotransferase 1 / ALT1 Rabbit Polyclonal Antibody

CAT#: AP21264BT-N

Alanine aminotransferase 1 / ALT1 rabbit polyclonal antibody, Biotin


USD 410.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
Working Dilutions:
Non-precipitating antibody-binding techniques: 1/2,000-1/50,000.
Reactivities Porcine
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Glutamic-Pyruvic Transaminase isolated and purified from porcine heart.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: Biotin
State: Lyophilized hyperimmune IgG fraction
Molar radio: Biotin/IgG ~ 4.2
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration 10 mg/ml
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Biotin
Background Alanine Transaminase [glutamic pyruvate transaminase - GPT] participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Pyridoxal phosphate is a known cofactor. Expression is induced by glucocorticoids. This enzyme is present in a high concentration in liver tissue and it's release into the blood indicates liver disease or damage.
Synonyms GPT, AAT1, GPT1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.