CRISP2 (81-93) Goat Polyclonal Antibody

CAT#: AP21265PU-N

CRISP2 (81-93) goat polyclonal antibody, Aff - Purified

Product Images

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 with C-terminal MYC tag probed with CRISP2 Antibody (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/8000.
  Western blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.5-1µg/ml. Primary incubations were for 1 hour. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml (Human Testis).
• Reactivities: Canine, Human, Porcine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to internal sequence amino acids 81-93 of Human CRISP2 (NP_003287.1).
• Specificity: Recognizes CRISP2.
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens cysteine rich secretory protein 2 (CRISP2), transcript variant 2
• Database Link:
  Entrez Gene 7180 Human
• Background: Cysteine-rich secretory protein 2 (CRISP2) may regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation. CRISP2 is specific to testis and epididymis.
• Synonyms: Cysteine-rich secretory protein 2, CRISP-2, Testis-specific protein TPX-1, Cancer/testis antigen 36, GAPDL5, TPX1, CT36

Reference Data

• Protein Families: Secreted Protein