ETFA (C-term) Goat Polyclonal Antibody

CAT#: AP21285PU-N

ETFA (C-term) goat polyclonal antibody, Aff - Purified

Product Images

ETFA antibody staining of Human Heart lysate at 0.1 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Limit Dilution: 1/8000.
  Western blot: 0.05-0.2 µg/ml. This antibody detects a band of ~30kDa in Human, Mouse and Rat Heart lysates.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_000117.1 and NP_001121188.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000117.1 and NP_001121188.1).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens electron transfer flavoprotein alpha subunit (ETFA), transcript variant 1
• Database Link:
  Entrez Gene 110842 MouseEntrez Gene 300726 RatEntrez Gene 2108 Human
• Background: ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Alpha-ETF
• Note: Calculated Molecular Weight: 30.0kDa (NP_001121188.1).

Reference Data

• Protein Families: Druggable Genome