SNAP23 Goat Polyclonal Antibody

CAT#: AP21296PU-N

SNAP23 goat polyclonal antibody, Aff - Purified

Product Images

SNAP23 antibody staining of Human Placenta lysate at 0.1ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: Limit Dilution: 1/8000.
  Western blot: 0.1-0.3 µg/ml. This antibody detects a band of ~26kDa Human Placenta lysates.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003816.2 and NP_570710.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_003816.2 and NP_570710.1).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Gene Name: Homo sapiens synaptosome associated protein 23kDa (SNAP23), transcript variant 1
• Database Link:
  Entrez Gene 20619 MouseEntrez Gene 64630 RatEntrez Gene 8773 Human
• Background: Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.
• Synonyms: SNAP-23
• Note: Calculated Molecular Weight: 23.4kDa (NP_003816.2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport