GPD1 Sheep Polyclonal Antibody

CAT#: AP21312AF-N

GPD1 sheep polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
  Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/100-1/900.
• Reactivities: Rabbit
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Glycerol-3-Phosphate Dehydrogenase isolated and purified from rabbit muscle.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The antibody recognizes Glycerol-3-Phosphate Dehydrogenase from Rabbit muscle.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Background: Glycerol 3 Phosphate Dehydrogenase is a homodimer and belongs to the NAD dependent Glycerol 3 Phosphate Dehydrogenase family.
• Synonyms: GPDH-C, GPDHC, GPD-C, GPDC, Glycerol-3-phosphate dehydrogenase