HXK1 Rabbit Polyclonal Antibody

CAT#: AP21320AF-N

HXK1 rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
  Recommended Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/1,000-1/20,000.
• Reactivities: Bakers Yeast
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Hexokinase isolated and purified from Baker’s Yeast.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Saccharomyces cerevisiae Hexokinase.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized Hyperimmune IgG fraction
• Reconstitution Method: Restore with 1.0 ml sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Background: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.
  In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase. Present with 40800 molecules/cell in log phase SD medium.
• Synonyms: HXK1, HKA, Hexokinase PI, Hexokinase-A