Hyaluronidase Rabbit Polyclonal Antibody

CAT#: AP21321BT-N

Hyaluronidase rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/2,000-1/10,000).
• Reactivities: Sheep
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Hyaluronidase isolated and purified from Sheep testes.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins.
  Label: Biotin
  State: Lyophilized IgG fraction.
  Molar radio: Biotin/IgG: ~1.0
• Reconstitution Method: Restore by adding 1.0 ml sterile distilled water.
• Concentration: 10 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
• Conjugation: Biotin
• Background: Hyaluronidase degrades hyaluronic acid, which is an important structural proteoglycan found in basement membranes and also extracellular matrices. There are six members of the hyaluronidase family. Hyaluronidase PH20 is a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane and plays a role in sperm penetration through the the hyaluronic acid-rich cumulus cell layer surrounding the oocyte. Abnormal expression of this gene has been implicated in degradation of basement membranes leading to tumor invasion and metastasis.
• Synonyms: HYAL1, HYAL2, LUCA1, LUCA-1, HYAL-1, Hyal-2, Hyaluronoglucosaminidase, Hyaluronidase