LDHA Sheep Polyclonal Antibody

CAT#: AP21338BT-N

LDHA sheep polyclonal antibody, Biotin


USD 410.00

2 Weeks*

Size
    • 1 ml

Product Images

Other products for "LDHA"

Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
Working Dilutions:
Non-precipitating antibody-binding techniques: 1/100-1/,000.
Reactivities Rabbit
Host Sheep
Isotype IgG
Clonality Polyclonal
Immunogen L-Lactic Dehydrogenase is isolated and purified from Rabbit muscle.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity L-Lactic Dehydrogenase from Rabbit muscle.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: Biotin
State: Lyophilized hyperimmune IgG fraction
Molar radio: Biotin/IgG ~ 6.6
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration 10 mg/ml
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Biotin
Background Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
Synonyms LDH-A, L-lactate dehydrogenase A chain, LDH-M, PIG19, NY-REN-59
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.