MDH1 Rabbit Polyclonal Antibody

CAT#: AP21344PU-N

MDH1 rabbit polyclonal antibody, Aff - Purified


USD 550.00

2 Weeks*

Size
    • 1 mg

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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
the customer’s own choice.
Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/3,000.
Reactivities Human
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Malic dehydrogenase is isolated and purified from Human placenta.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
State: Aff - Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore by adding 0.5 ml of sterile distilled water
Concentration lot specific
Purification Solid Phase Affinity Chromatography
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2
Background Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.
Synonyms MDHA, Malate dehydrogenase, cytoplasmic
Reference Data
Protein Families Druggable Genome
Protein Pathways Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.