Phospholipase C / PLC Rabbit Polyclonal Antibody

CAT#: AP21376AF-N

Phospholipase C / PLC rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.
  Recommended working dilutions: vary widely, but may be between 1/1000 and 1/20000.
• Reactivities: Bacillus sp.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phospholipase C isolated and purified from Bacillus cereus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: IgG fraction of polyclonal rabbit antiserum to phospholipase C from Bacillus cereus.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10,0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Synonyms: Cereolysin A