PHKB Sheep Polyclonal Antibody

CAT#: AP21381AF-N

PHKB sheep polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.
  Working dilutions: vary widely, but may be between 1/100 and 1/700.
• Reactivities: Rabbit
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phosphorylase b isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: IgG fraction of polyclonal sheep antiserum to phosphorylase b from rabbit muscle.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized Hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10,0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Database Link:
  Entrez Gene 100009137 Rabbit
• Synonyms: DKFZp781E15103; FLJ41698