PHKB Sheep Polyclonal Antibody

CAT#: AP21381BT-N

PHKB sheep polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
  Working dilutions: vary widely, but may be between 1/200 and 1/2000.
• Reactivities: Rabbit
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phosphorylase b isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Biotin-conjugated IgG fraction of polyclonal sheep antiserum to phosphorylase b from rabbit muscle.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized Hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~5.8
• Reconstitution Method: Restore with 1,0 ml distilled water
• Concentration: 10,0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Database Link:
  Entrez Gene 100009137 Rabbit
• Synonyms: DKFZp781E15103; FLJ41698