Proteinase K / PROK Rabbit Polyclonal Antibody

CAT#: AP21385AF-N

Proteinase K / PROK rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer's own choice.
  Recommended Working Dilutions: vary widely, but may be between 1/10000 and 1/1000000. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Proteinase K isolated and purified from Tritirachium album. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: IgG (7S) fraction of polyclonal Rabbit antiserum to Proteinase K from Tritirachium album.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized Hyperimmune purified IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Synonyms: Endopeptidase K, Tritirachium alkaline proteinase