Fibrinogen Goat Polyclonal Antibody

CAT#: AP21470SU-N

Fibrinogen goat polyclonal antibody, Texas Red


USD 270.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Applications ID, IP
Recommended Dilution In precipitating techniques as Immunoelectrophoresis and Single or Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of Fibrinogen in Mouse plasma or other body fluids or to determine its concentration.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal Mouse plasma in agar-block immunodiffusion titration.
Reactivities Mouse
Host Goat
Clonality Polyclonal
Immunogen Fibrinogen is isolated from fresh plasma after removing Prothrombin.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reactivity of the antiserum is restricted to Mouse fibrinogen molecule.
In Immunoelectrophoresis and Radial Immunodiffusion, using various antiserum concentrations against normal Mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified Fibrinogen. No reaction is obtained with any other plasma protein components or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products.
The antiserum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation Label: Texas Red
State: Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives
Label: Labeled with Texas Red
Reconstitution Method Restore by adding 1 ml of sterile distilled water.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Conjugation Texas Red
Background Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking.
Synonyms FGA, FGB, FGG
Note Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Reference Data

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