DLL4 Rabbit Polyclonal Antibody

CAT#: AP21750BT-N

DLL4 rabbit polyclonal antibody, Biotin


USD 355.00

2 Weeks*

Size
    • 50 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Human sDLL-4 by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant hsDLL-4. 
Sandwich ELISA:
 To detect Human sDLL-4 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Affinity purified Anti-Human sDLL-4 (Cat.-No AP21750PU-N or AP21750PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sDLL-4. 
Western Blot: To detect Human sDLL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsDLL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure HEK293 cells derived Recombinant Human sDLL-4 (Cat.-No AR31113PU-N).
Specificity Recognizes Human sDLL-4. Other species not tested.
Formulation PBS, pH 7.2 without preservatives
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method

Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Purification Affinity Chromatography
Conjugation Biotin
Gene Name Homo sapiens delta-like 4 (Drosophila) (DLL4)
Background

DLL4 is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

Synonyms Delta 4, Drosophila Delta homolog 4
Reference Data
Protein Families Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
Protein Pathways Notch signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.