Cytochrome P450 2E1 (CYP2E1) Goat Polyclonal Antibody

CAT#: AP22383PU-N

Cytochrome P450 2E1 (CYP2E1) goat polyclonal antibody, Aff - Purified

Product Images

CYP2E1 antibody staining of Human Liver lysate at 0.05ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  CYP2E1 antibody staining of paraffin embedded Human Liver at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/32000 ( Detection Limit).
  Western blot: 0.05-0.2 µg/ml. Detects a band of ~50kDa in Human Liver lysates.
  Immunohistochemistry on Paraffin Sections: 3-5 µg/ml. In paraffin embedded Human Liver shows ER-like staining in hepatocytes.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000764.1.
• Specificity: Recognizes Human CYP2E1.
  Other species not tested.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1571 Human
• Background: The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and biosynthesis. Cytochrome P450 2E1 is induced by ethanol, diabetes and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, and exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
• Synonyms: Cytochrome P450 2E1, CYP2E, CYPIIE1, Cytochrome P450-J
• Note: Calculated Molecular Weight: 56.8kDa (NP_000764.1).