SNAIL (SNAI1) (N-term) Goat Polyclonal Antibody

CAT#: AP22444PU-N

SNAIL (SNAI1) (N-term) goat polyclonal antibody, Aff - Purified

Product Images

AP22444PU-N (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  AP22444PU-N (0.1ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  AP22444PU-N (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: Limit Dilution: 1/128000.
  Western Blot: 0.1-0.3 µg/ml. Approx 28kDa band observed in lysates of cell line A431 and in Rat Kidney lysates (calculated MW of 29.1kDa according to Human NP_005976.2 and Rat NP_446257.1).
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_005976.2.
• Specificity: Snail homolog 1 / SNAI1
• Formulation: Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 6615 Human
• Background: Zinc finger protein SNAI1 (SNAIL) is a zinc finger transcriptional repressor which down regulates the expression of ectodermal gene within the mesoderm and thus functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stroma may be a new putative prognosis marker for colon tumors. In recent studies SNAI1 is found to be potential target for proteasome degradation by hypoxia-controlled FBXL14 ubiquitin ligase targets.
• Synonyms: SNAH, Protein snail homolog 1