Frizzled 2 (FZD2) Goat Polyclonal Antibody

CAT#: AP22449PU-N

Frizzled 2 (FZD2) goat polyclonal antibody, Aff - Purified


USD 375.00

5 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, IF, IHC, WB
Recommended Dilution Peptide ELISA: Limit Dlution: 1/64000.
Western Blot:  0.03-0.1 µg/ml. Approx 60-65kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24kDa was also observed in some lysates which was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
Immunohistochemistry on Paraffin Sections:
2-5 µg/ml in paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles.
2.5 µg/ml in paraffin embedded Human Liver. 
Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells.
Reactivities Human, Mouse, Rat
Host Goat
Clonality Polyclonal
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_001457.1.
Specificity Recognizes FZD2 / Frizzled-2.
Formulation Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
State: Aff - Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens frizzled class receptor 2 (FZD2)
Background FZD2 is a Frizzled Receptor that mediates Wnt signaling, which is required for proper axis development in the embryo. FZD2 is developmentally regulated. Studies of rat FZD2 indicate that the protein signals through a calcium-releasing pathway and that FDZ2 expression modulates protein kinase C (PKC) localization and stimulates PKC activity in vitro. Increased levels of FZD2 mRNA have been observed in primary gastric cancer and in head and neck squamous carcinoma and gastric cancer cell lines.
FZD2 expression has been documented in human brain, heart, kidney, lung, colon, ovary and stomach. High levels of expression have been observed in fetal kidney and lung and adult colon and ovary. ESTs have been isolated from human tissue libraries, including cancerous kidney, lung, lymph and testis, and normal blood, cervix, embryo and kidney.
Synonyms Fz-2, hFz2, FzE2
Reference Data
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway

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