ROBO1 (994-1006) Goat Polyclonal Antibody

CAT#: AP22501PU-N

ROBO1 (994-1006) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Liver (formalin-fixed, paraffin-embedded) stained with ROBO1 antibody at 2 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/8000.
  Immunohistochemistry on Paraffin Sections: 2 µg/ml.
  Western Blot: 1 - 3 µg/ml.
• Reactivities: Bat, Bovine, Canine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human ROBO1
• Specificity: This antibody detects ROBO1 at aa 994-1006.
  It is expected to recognise all reported isoforms (NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant 1
• Database Link:
  Entrez Gene 19876 MouseEntrez Gene 58946 RatEntrez Gene 6091 Human
• Background: Robo proteins are highly conserved transmembrane receptors that are expressed by growing axon tips. They play multiple parts in guiding axons to their correct locations in the developing brain and are particularly important for enabling axons to cross the midline. In all mammalian species studied so far, Robo is the receptor for Slit, a protein secreted by the midline of the nervous system. When bound to each other, Split and Robo act as a chemorepellent, inducing growth of axon tips away from the midline.
• Synonyms: ROBO-1, Roundabout homolog 1, DUTT1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance