CD14 (71-84) Goat Polyclonal Antibody

CAT#: AP22502PU-N

CD14 (71-84) goat polyclonal antibody, Aff - Purified

Product Images

(1 ug/ml) Staining of Human Lymph Node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Spleen (formalin-fixed, paraffin-embedded) stained with CD14 antibody at 4 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/128000.
  Immunohistochemistry on Paraffin Sections: 4 µg/ml.
  Western Blot: 1 - 3 µg/ml.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human CD14 (NP_000582.1)
• Specificity: This antibody detects CD14 at aa 71-84.
• Formulation: Tris buffered saline, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD14 molecule (CD14), transcript variant 1
• Database Link:
  Entrez Gene 929 Human
• Background: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
• Synonyms: CD14

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, MAPK signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Toll-like receptor signaling pathway