WNT3 (315-329) Goat Polyclonal Antibody

CAT#: AP22539PU-N

WNT3 (315-329) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Adrenal (formalin-fixed, paraffin-embedded) stained with WNT3 antibody at 3.75 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Breast (formalin-fixed, paraffin-embedded) stained with WNT3 antibody at 3.75 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Thyroid (formalin-fixed, paraffin-embedded) stained with WNT3 antibody at 3.75 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/2000.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
  Western Blot: 1/2000, 1 - 3 µg/ml.
• Reactivities: Bat, Bovine, Canine, Chicken, Equine, Human, Monkey, Mouse, Porcine, Rat, Xenopus, Zebrafish
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human WNT3
• Specificity: This antibody detects WNT3 (Internal).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens wingless-type MMTV integration site family member 3 (WNT3)
• Database Link:
  Entrez Gene 22415 MouseEntrez Gene 24882 RatEntrez Gene 7473 Human
• Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product.
• Synonyms: Proto-oncogene protein Wnt-3, INT4, INT-4

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway