TCF3 / E2A (TCF3) (522-535) Goat Polyclonal Antibody

CAT#: AP22543PU-N

TCF3 / E2A (TCF3) (522-535) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (0.01 ug/ml) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Breast (formalin-fixed, paraffin-embedded) stained with TCF3 antibody at 3.75 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Kidney (formalin-fixed, paraffin-embedded) stained with TCF3 antibody at 3.75 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/128000.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
  Western Blot: 1/128000, 0.01 - 0.03 µg/ml.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human TCF3
• Specificity: This antibody detects TCF3 / E2A (Internal).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens transcription factor 3 (TCF3), transcript variant 2
• Database Link:
  Entrez Gene 6929 Human
• Background: TCF3 / E2A is a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development.
• Synonyms: Transcription factor 3, TCF-3, Transcription factor E2-alpha, ITF1, ITF-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors