COX4 (COX4I1) (154-166) Goat Polyclonal Antibody

CAT#: AP22551PU-N

COX4 (COX4I1) (154-166) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (0.01 ug/ml) staining of human muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Testis (formalin-fixed, paraffin-embedded) stained with COX4I1 antibody at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Thyroid (formalin-fixed, paraffin-embedded) stained with COX4I1 antibody at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/4000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/4000, 0.01 - 0.03 µg/ml.
• Reactivities: Human, Monkey
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C-term of human COX4I1
• Specificity: This antibody detects COX IV (C-term).
  It is expected to recognize COX4I1 (GeneID:1327; MW: 19.6kDa) & COX4I2 (GeneID:84701, MW:19.6kDa).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens cytochrome c oxidase subunit 4I1 (COX4I1), transcript variant 1
• Database Link:
  Entrez Gene 1327 Human
• Background: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities.
• Synonyms: Cytochrome c oxidase polypeptide IV, COX IV-1, COX4I1, COX4

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease