PPP4C (290-301) Goat Polyclonal Antibody

CAT#: AP22586PU-N

PPP4C (290-301) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (0.3 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Small Intestine (formalin-fixed, paraffin-embedded) stained with PPP4C antibody followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Prostate (formalin-fixed, paraffin-embedded) stained with PPP4C antibody followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 0.1 - 0.3 µg/ml.
• Reactivities: Bat, Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebrafish
• Host: Goat
• Clonality: Polyclonal
• Immunogen: synthetic peptide from the C-terminus of human PPP4C (NP_002711.1)
• Specificity: This antibody detects PPP4C at aa 290-301.
• Formulation: Tris saline buffer, pH7.3 containing 0.02% sodium azide as preservative and 0.5% bovine serum albumin as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens protein phosphatase 4 catalytic subunit (PPP4C), transcript variant 2
• Database Link:
  Entrez Gene 56420 MouseEntrez Gene 171366 RatEntrez Gene 5531 Human
• Background: Protein phosphatase 4 (protein phosphatase X) is a 35kD serine/threonine phosphatase with predicted binding sites for iron and manganese. It is found in both the cytoplasm and the nucleus. The phophoprotein alpha 4 has been identified as a potential regulatory subunit. PPP4C has been shown to localize to centrosomes/spindle pole bodies and is believed to be involved in microtubule organization. It may also play a role in relaying the TNF-alpha signal to the JNK pathway. Additionally, it has been shown to associate with C-Rel, NF-Kappa B p50, and RelA. Hence, it may be involved in the activation of these key mediators of immune response and apoptosis.
• Synonyms: PPP4, PP4C, PPX, PP-X, Protein phosphatase X

Reference Data

• Protein Families: Druggable Genome, Phosphatase