Lamin A (LMNA) (N-term) Rabbit Polyclonal Antibody

CAT#: AP22639PU-N

Lamin A (N-Terminus) rabbit polyclonal antibody, Affinity purified

Product Images

IHC Image for AP22639PU-N Human Skin (formalin-fixed, paraffin-embedded) stained with Lamin C antibody AP22639PU-N at 1/50 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF
  IHC
  IHC-Fr
  IHC-P (1:50)
  WB
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A peptide made from the last 8 amino acids of Lamin C, including an N-Terminus lysine as a linker (KHHVSGSRR), coupled to KLH
• Specificity: This antibody reacts strongly in immunoblotting with fragments corresponding to peptide 319-572aa of Lamin C. When tested against nuclear protein extracts from HeLa cells it reacts with a single band migrating at 65 kD. Stains Lamin C from human, mouse and rat material
• Formulation: PBS, 0.1% Sodium Azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
• Stability: 12 months from date of despatch
• Gene Name: Homo sapiens lamin A/C (LMNA), transcript variant 2
• Database Link:
  Entrez Gene 16905 MouseEntrez Gene 60374 RatEntrez Gene 4000 Human
  UniProt ID P02545
• Background: Lamin C antibody LS-B2972 is an unconjugated rabbit polyclonal antibody to Lamin C (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
• Synonyms: CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; OTTHUMP00000015843; PRO1
• Note: Immunohistochemistry: LS-B2972 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2972 was determined to be 1:50.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)