APAF1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP22708PU-N

APAF1 (C-term) rabbit polyclonal antibody, Purified

Product Images

Human testis stained with Anti-Apaf-1 antibody (AP22708PU-N)

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  Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with APAF1 antibody AP22708PU-N at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Heart (formalin-fixed, paraffin-embedded) stained with APAF1 antibody AP22708PU-N at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Spleen (formalin-fixed, paraffin-embedded) stained with APAF1 antibody AP22708PU-N at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 10 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: APAF1 antibody was raised against a synthetic peptide derived from the C-terminus of human Apaf-1
• Specificity: This antibody reacts to Apoptotic Protease Activating Factor 1 (APAF1).
• Formulation: 10 mM PBS, pH 7.4, BSA, sodium azide.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2
• Database Link:
  Entrez Gene 11783 MouseEntrez Gene 78963 RatEntrez Gene 317 Human
• Background: Apaf1 has been described as the core of the apoptosome. Deficiency in murine Apaf1 leads to embryonic lethality with a phenotype affecting many aspects of developmental apoptosis. In the developing brain, Apaf1 is a death regulator of the neuronal founder cells. Combined intercrosses of mouse lines mutant for members of the mitochondrial death pathway are providing us with some clues about the relative regulation existing among neuronal cell populations. Apaf1-deficient embryos display an interesting phenotype in the inner ear and in limb development, which involves different caspase-dependent and -independent pathways. Moreover, APAF1 is mutated in human melanomas, and its depletion contributes to malignant transformation in a mouse model of cancer.
• Synonyms: Apaf-1, KIAA0413

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Huntington's disease, p53 signaling pathway, Parkinson's disease, Small cell lung cancer