FLIP (CFLAR) (191-209) Rabbit Polyclonal Antibody

CAT#: AP22849PU-N

FLIP (CFLAR) (191-209) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of FLIPg/d in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIPg/d antibody at 1/1000 dilution.

• 

  Human Lung (formalin-fixed, paraffin-embedded) stained with CFLAR antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Human Pancreas (formalin-fixed, paraffin-embedded) stained with CFLAR antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Human Placenta (formalin-fixed, paraffin-embedded) stained with CFLAR antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Immunocytochemical staining of HeLa cells using FLIPg/d antibody at 2 ug/ml.

Show all 5 images

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunocytochemistry.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: CFLAR antibody was raised against synthetic peptide
• Specificity: This antibody reacts to CASP8 And FADD-like Apoptosis Regulator (CFLAR).
• Formulation: PBS containing 0.02% sodium azide.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1 mg/ml
• Purification: Immunoaffinity Chromatography
• Gene Name: Homo sapiens CASP8 and FADD like apoptosis regulator (CFLAR), transcript variant 2
• Database Link:
  Entrez Gene 12633 MouseEntrez Gene 8837 Human
• Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively.
• Synonyms: c-FLIP, CLARP, MRIT, CASH, FLAME-1, CASP8AP1, Usurpin

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis