PHAP1 (ANP32A) (N-term) Rabbit Polyclonal Antibody

CAT#: AP22856PU-N

PHAP1 (ANP32A) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of PHAP expression in human Raji cell lysate with PHAP antibody (AP22856PU-N) at 1 ug/ml. The wide and strong band below PHAP III is a doublelet composed of PHAP I (upper) and PHAP I2a (lower).

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  Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ANP32A antibody AP22856PU-N at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Kidney (formalin-fixed, paraffin-embedded) stained with ANP32A antibody AP22856PU-N at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Liver (formalin-fixed, paraffin-embedded) stained with ANP32A antibody AP22856PU-N at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunocytochemistry of PHAP in Raji cells with PHAP antibody (AP22856PU-N) at 1 ug/ml.

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunocytochemistry: 1 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 0.5 - 2 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: ANP32A antibody was raised against synthetic peptide
• Specificity: This antibody reacts to Acidic (leucine-rich) Nuclear Phosphoprotein 32 Family, Member A (ANP32A/PHAP1).
• Formulation: PBS containing 0.02% sodium azide.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1 mg/ml
• Purification: Immunoaffinity Chromatography
• Gene Name: Homo sapiens acidic nuclear phosphoprotein 32 family member A (ANP32A)
• Database Link:
  Entrez Gene 11737 MouseEntrez Gene 25379 RatEntrez Gene 8125 Human
• Background: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
• Synonyms: C15orf1, LANP, MAPM, PHAP1, Mapmodulin

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency