Myosin Phosphatase (PPP1R12A) Goat Polyclonal Antibody

CAT#: AP22981PU-N

Myosin Phosphatase (PPP1R12A) goat polyclonal antibody, Aff - Purified

Product Images

Skeletal muscle, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
• Reactivities: Bat, Bovine, Canine, Equine, Human, Monkey, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from human PPP1R12A / MYPT1
• Specificity: This antibody reacts to Myosin Phosphatase, Target Subunit 1 (MYPT1) (PPP1R12A).
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA and 0.02% sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant 2
• Database Link:
  Entrez Gene 17931 MouseEntrez Gene 116670 RatEntrez Gene 4659 Human
• Background: Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
• Synonyms: MBS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction